Tag Archives: HL60

Dicty World Race 2014 Winners Announced!

The following report is taken from Dicty World Race organizer, Daniel Irimia’s lab note on Experiment.

Dicty Cells take 1st place!

The winners of the first Dicty World Race 2014 have been announced.. Dicty cells submitted by the lab of Arjan Kortholt and Peter van Haastert from U. Groningen, Netherlands have taken first place. These cells were trained by Ineke Keizer-Gunnink and Rama Kataria. The winner cells accounted for a remarkable 60% of the cells in the top 100 to finish the race.

… With HL60 cells in 2nd place!

In second place, are the HL60 cells from the group of Guillaume Charras in London, UK.

Other Top Finishers:

1.  A. Kortholt and P. van Haastert lab (Dicty) 
2.  G. Charras lab (HL60) 
3.  J. Faix lab (Dicty) 
4.  M. Myre lab (Dicty) 
5.  A. Kimmel lab (Dicty) 
6.  T. Bruce lab (Dicty) 

Read the full report, here.

See the forecast trends and history for the question: Which team will win the 2014 Dicty World Race? 

In addition to the questions for each specific team’s placement, SciCast asked which team will enter the smartest and the fastest cells. All Dicty Race questions have been paused. If you made forecasts, login and then visit your dashboard to see how you did. Watch for the SciCast analysis, coming soon.

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Second Dicty Amateur Video Analysis (17-May)

** Updated 18-May **

Some videos have been added today (refresh https://scicast.org/dicty), so I took a second look.  I don’t look through microscopes for a living, so this isn’t final.  Times below are the timestamped clock time, which elapses at about 8m per frame. I collected:

  • TeamNum: Team name number stated in the video title
  • TeamName: Team name and/or PI name(s)
  • t_First: time when the first cell entered the finish zone. There is some ambiguity for cells that hang out on the opening.
  • Num_3hr: #cells in the finish zone by the 3hr mark; not sure how Irimia is counting cells that leave after entering
  • Num_5hr: #cells in the finish zone by the 5hr mark (only 4 videos)
  • Notes: salient notes, in quotes

Often there were two mazes and finish lines visible.  In these cases I tried to count both finish lines.  I’m not sure that’s canonical.  Here are the results as I saw them (Changed format to avoid margin clipping; sorted by Num_3hr):

TeamNum,TeamName,t_First,Num_3hr,Num_5hr,Notes
Team 12,Kortholt,1:36,14,,”3 in at 1:36,4 @ 1:40,at least 14 at 3h”
Team 11,Faix,1:28,12,,3 in @ 1:36; I counted at least 12 entering by 3h
Control HL60,,1:42,9,,9+. I can’t get it to hold the last frame to count.
Team 9 HL60,Insall,2:14,5,,5+. Many cells got bored and went back into the maze.
Team 17,Kimmel,1:36,5,,5+. Can’t hold the last frame.
Caffeine Control,,1:56,4,,Last one just in time.
Race2Control,,2:15,2,5,”Lots of cells,lots of movement,esp. outside the maze.”
Racer X Neutrophils,,2:25,2,,Cell entering 2:25 jumps track next frame 2:30. Entered?
Team1,Strassman & Queller,2:38,1.5,3,Video starts at 1:30; 2nd cell just crossing at 3:00
Team 9,Insall,,0,0,”Video starts at 1:30; hard to see,but cells appear disorganized.”
Team18,Kay,4:07,0,1,Cells outside the maze are much faster!
Team5 HL60,Steinckwich-Besancon,,0,,”Only 5-6 cells visible,hardly moving.”
Team 19 HL60,Tschirhart,,0,,17 cells idly drifting around the mazes.
Team 7,Bruce,,0,,Halfway through the maze at 3h.
Team 10,Beta,,0,,No cells visible on the slide.
Team 20,Devreotes,,0,,Only 1 confused cell. Did preliminary videos have the wrong label?
Team 9,Insall,,0,,About halfway through at 3h.
Team 15,Myre,,0,,About halfway through at 3h.
Team14,Muller-Taubenberger,,,,Irimia says cells did not get to the starting line.

So, likely winners:

  • Overall winner (most finishers at 3h): Team 12 (Kortholt) with 14+ cells.  We had an email from Irimia mid-afternoon saying at that point they also thought 12 was the most likely.  Second place is probably Team 11 (Faix) with 12+ cells in. Third is likely to be either the HL60 line from Team 9 (Insall), or Team 17 (Kimmel), both with at least 5 cells in by 3hr.
  • Fastest: Team 11 (Faix) just edges out Teams 12 (Kortholt) and 17 (Kimmel) for the fastest single cell at 1:28. This may turn on the precise definition of finish line.
  • Smartest: Sorry, I don’t have the patience to count wrong turns.

Notes: Neutrophils need to be photographed more often — the fastest ones are simply sprinting too fast to uniquely infer motion between frames.

You can still edit the Fastest and Smartest questions.  Until Irimia announces the winners anyway.   https://scicast.org/dicty.

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Prepped and Loaded – Cells Loaded into the Maze

The cells have been loaded into the maze. The final preparations for the official start are wrapping up… We’re getting so close to seeing what these cells can really do!

Loading HL60 cell lines into maze

Loading HL60 cell lines into maze

HL60 cells under the microscope

HL60 cells under the microscope

The microscope is motorized so it cycles through the spots on the slide every 5 minutes and takes a picture. The pictures are strung together to make the final "movie" of the race

The microscope is motorized so it cycles through the spots on the slide every 5 minutes and takes a picture. The pictures are strung together to make the final “movie” of the race

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